69 / 453
5
(a)
3M Molecular Detection System
(MDS100) – Available from 3M Food Safety (St. Paul, MN 55144-
1
1000, USA).
2
(b)
3M Molecular Detection Assay 2 – E. coli
O157 (including H7)
Catalog number:
3
MDA2ECO96,
reagent tubes-
12 strips of 8 tubes. Available from 3M Food Safety (St. Paul, MN
4
55144-1000, USA).
5
(c)
Lysis Solution (LS) tubes –
12 strips of 8 tubes.
6
(d)
Extra caps
– 12 strips of 8 caps
7
(e)
Reagent Control –
8 reagent tubes
8
(f)
Quick Start Guide
9
(g)
3M
™
Molecular Detection Speed Loader Tray -
Available from 3M Food Safety
0
(St. Paul, MN 55144-1000, USA).
1
(h)
3M
™
Molecular Detection Chill Block Insert
- Available from 3M
™
Food Safety (St. Paul, MN 55144-
2
1000, USA).
3
(i)
3M
™
Molecular Detection Heat Block Insert
- Available from 3M Food Safety
4
(St. Paul, MN 55144-1000, USA).
5
(j)
3M
™
Molecular Detection Cap/Decap Tool for Reagent tubes
- Available from 3M Food Safety (St.
6
Paul, MN 55144-1000, USA).
7
(k)
3M
™
Molecular Detection Cap/Decap Tool for Lysis tubes
– Available from 3M Food Safety (St. Paul,
8
MN 55144-1000, USA).
9
(l)
Empty Lysis Tube Rack
- Available from 3M Food Safety (St. Paul, MN 55144-1000, USA).
0
(m)
Empty Reagent Tube Rack
- Available from 3M Food Safety (St. Paul, MN 55144-1000, USA).
1
(n)
Buffered Peptone Water (ISO Formulation)
– Available from 3M Food Safety (St. Paul, MN 55144-
2
1000, USA). (Formulation equivalent to ISO 6579:2002 Annex B or 3M equivalent)
3
(o)
Disposable pipette
– capable of 20 µL
4
(p)
Multi
-
channel (8-channel) pipette
- capable of 20 µL
5
(q)
Sterile filter tip pipette tips
- capable of 20 µL
6
(r)
Filter Stomacher® bags –
Seward or equivalent
.
7
(s)
Stomacher
®– Seward or equivalent.
8
(t)
Partial immersion thermometer –
calibrated range to include 100 ± 1
o
C
9
(u)
Dry block heater unit–
capable of maintaining 100 ± 1
o
C
0
(v)
Incubators.
– Capable of maintaining 37 ± 1°C or 41.5 ± 1°C.
1
(w)
Refrigerator –
capable of maintaining 2-8
o
C, for storing the 3M Molecular Detection Assay
2
components
3
(x)
Computer –
compatible with the 3M
™
Molecular Detection Instrument
4
(y)
Modified TSB+Novobiocin –
Available from Mediabox™ Actero™ (Calgary, Alberta, Canada T2R
5
1J6)
6
(z)
OctoMACS™
7
(aa)
Multistand to support OctoMACS
8
(bb)
MACS
®
Large Cell Separation Columns
-Available from MiltenyBiotec (Gaithersburg, MD, 20878,
9
USA)
0
(cc)
Dynabeads
®
anti-E. coli O157
(Dynal Inc., Lake Success, NY 11042, USA)
1
(dd)
Tryptic Soy Agar with 5% sheep blood (SBA)
-(TS-BD, Franklin Lakes, New Jersey 07417, USA;
2
Sheep blood-Quad Five, Ryegate, Montana 59074, ,USA)
3
(ee)
Rainbow® Agar O157, Dehydrated
(Biolog Inc., Hayward California, 94545, USA)
4
(ff)
Novobiocin (5.0 mg/L)
(Alfa Aesar, Tewksbury, MA 01876, USA)
5
(gg)
Cefixime (0.05 mg/L)
(Sigma Aldrich, St. Louis, MO 63103, USA)
6
(hh)
Potassium Tellurite (
0.15 mg/L
)
(Oxoid, Hampshire RG24 8PW, UK)
7
(ii)
1N HCL
(Acros, New Jersey, USA)
8
OMAMAN-35 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only