24
New Biological Frontiers Illuminated by Molecular Sensors and Actuators
Tuesday Speaker Abstracts
Sensing and Structure Investigations of Nucleic Acid Systems Using Fluorescent Base
Analogue FRET-Probes
Marcus Wilhelmsson
.
Chalmers University of Technology, Gothenburg, Sweden.
See abstract: Pos-47 Board 47
Actuators within Sensors: The Architecture of Primary Cilia
Jung-Chi Liao
, T T. Yang.
Academia Sinica, Taipei, Taiwan.
Primary cilia are cellular sensors associated with important signaling pathways including
hedgehog signaling and Wnt signaling. Intraflagellar transport is actively regulated to deliver
precursors and other molecules in primary cilia. Despite the importance, our knowledge of the
ciliary architecture is limited, hindering a structure-based understanding of ciliary functions.
Here we reveal the molecular architecture at the base of primary cilia using superresolution
microscopy. We found that there are multiple levels of trafficking rests for intraflagellar
transport proteins and transmembrane proteins, suggesting the gating regulation at the ciliary
base is performed at different resting sites upon the architecture of primary cilia.