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New Biological Frontiers Illuminated by Molecular Sensors and Actuators
Poster Abstracts
18-POS
Board 18
Fluorescent Nucleic Acid Systems: Design, Construction and Biosensing
Byeang Hyean Kim
.
POSTECH, Pohang, Gyeong-buk, South Korea.
Sequence specific hybridization probes composed of fluorescent synthetic oligonucleotides have
been developed for genetic analysis in the post-genomic era. Currently such fluorescent
oligonucleotides play a decisive role in analysis of the genetic information and DNA sensing
such as SNP typing.
Fluorescent nucleic acid systems are widely applied in various fields, from fundamental
biological probes to nano-construction, Nucleic acids are used as a scaffold for arranging
aromatic fluorophore assemblies, either by insertion into the DNA base pairs or by stacking via
the duplex. Moreover, chemical modifications of nucleic acids are accessible by the modified
DNA phosphoramidites or postsynthetic approach, and provide with new and interesting
fluorescent nucleic acids systems. Fluorescent nucleic acid systems represent an extensive and
exciting research area in chemistry as well as in biotechnology and photophysics. We have
synthesized and investigated new fluorescent nucleic acid systems for probing single nucleotide
polymorphisms (SNPs), structural changes of DNA4 and ligand interaction with RNA bulge.
We have developed the new type of molecular beacon, quencher-free molecular beacon (QF-
MB), that exhibits several advantageous features, including a high level discrimination between
the target and its single-mismatched congeners and an economical device set-up due to the
absence of the quencher. We have also designed and synthesized the probing system for
quadruplex structures of DNA (G-quadruplex and i-motif) and B-Z transition.
Strong π–π stacking interactions in nucleic acids can be used to generate novel secondary
structures. We have investigated the fluorescent phenomena and structures of pyrene modified
oligodeoxyadenylate and oligodeoxyguanylate. The covalently linked pyrenes induced the
formation of a self-assembled olgiodeoxyadenylate duplex5 and various secondary structures
with interesting fluorescence phenomena. Various results using fluorescent nucleic acid systems
will be discussed.