AOAC ISPAM Meeting eBook, March 17 2015

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Draft, Do Not Distribute
Annex II: Inclusivity Panel
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The inclusivity panel shall include:
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• Sequences from at least two representative strains from each major clade
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of Variola virus
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• Any other strain with differences in the assay primer and/or probe target
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sequences based on bioinformatic analysis (Annex V).
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Note: The World Health Organization (WHO) restricts access to Variola virus
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genomic material; use of any genomic sequences greater than 500 bp requires
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written permission/approval from the WHO. Insertion of Variola virus DNA into
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other Orthopoxviruses is prohibited.
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More details can be found at:
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5 Approved Variola SMPR v7.5