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Draft, Do Not Distribute
Part 2: Environmental Panel Organisms -
This list is comprised of identified organisms from the
144
environment.
145
146
Inclusion of all environmental panel organisms is not a requirement if a method developer provides
147
appropriate justification that the intended use of the assay permits the exclusion of specific panel
148
organisms. Justification for exclusion of any environmental panel organism(s) must be documented
149
and submitted.
150
151
Organisms and cell lines may be tested as isolated DNA, or as pools of isolated DNA. Isolated DNA
152
may be combined into pools of up to 10 panel organisms, with each panel organism represented at
153
10 times the AMDL, where possible. The combined DNA pools are tested in the presence (at 2 times
154
the AMDL) and absence of the
target viral gene or gene fragment.
If an unexpected result occurs,
155
each of the individual environmental organisms from a failed pool must be individually re-tested at
156
10 times the AMDL with and without the
target viral gene or gene fragment
at 4,000 genome
157
equivalents/mL in the candidate method DNA elution buffer.
158
159
160
161
•
Other biothreat agents
162
163
Bacillus anthracis
Ames
164
Yersinia pestis
Colorado-92
165
Francisella tularensis
subsp.
tularensis
Schu-S4
166
Burkholderia pseudomallei
167
Burkholderia mallei
168
Coxiella burnetii
169
Brucella melitensis
170
Ricinus communis
– use ricin plant leaves as source of DNA
171
Clostridium botulinum
Type A
172
173
•
Cultivatable bacteria identified as being present in air and soil
174
Acinetobacter lwoffii
175
Agrobacterium tumefaciens
176
Bacillus amyloliquefaciens
177
Bacillus cohnii
178
Bacillus psychrosaccharolyticus
179
Bacillus benzoevorans
180
Bacillus megaterium
181
Bacillus horikoshii
182
Bacillus macroides
183
Bacteroides fragilis
184
Burkholderia cepacia
185
Burkholderia gladoli
186
Burkholderia stabilis
187
Burkholderia plantarii
188
Chryseobacterium indologenes
189
Clostridium sardiniense
190
Clostridium perfringens
191
8 Approved
Variola
SMPR v7.5