114-115 / 471
Table 8. Total Yeast and Mold Log
10
counts for raw almonds by 3M Petrifilm RYM Count Plate
after 48 hours incubation at 28
o
C vs. FDA BAM and ISO 21527 reference methods
a
Uninoculated Control
Low
Medium
High
Petrifilm
RYM
FDA BAM/
ISO 21527
g
Petrifilm
RYM
FDA BAM/
ISO 21527
g
Petrifilm RYM
FDA BAM/
ISO 21527
g
Petrifilm
RYM
FDA BAM/
ISO 21527
g
Lab
A
b
B
A
B
A
B
A
B
A
B
A
B
A
B
A
B
1
1.00
e
1.00
e
1.00
e
<1.00
1.30
1.48
1.78
1.85
1.78
1.90
2.28
1.95
1.90
d
2.20
d
2.04
c
2.40
c
2
1.30
e
1.30
e
1.30
e
1.85
e
1.78
1.60
2.04
1.70
1.78
1.78
2.11
2.34
2.11
d
2.11
d
2.64
2.84
3
<1.00
<1.00
<1.00
<1.00
1.78
1.30
1.60
1.60
2.34
2.30
2.38
2.18
3.57
3.15
3.30
3.11
4
f
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
5
<1.00
<1.00
<1.00
<1.00
1.60
1.78
1.78
1.95
2.30
2.00
2.30
2.45
3.34
3.15
3.18
3.18
6
<1.00
<1.00
<1.00
<1.00
1.78
1.30
2.20
1.78
1.78
1.30
2.34
2.20
3.04
3.11
3.36
3.28
7
<1.00
<1.00
<1.00
<1.00
1.60
1.48
1.70
1.30
2.36
2.20
2.43
2.11
3.18
3.04
3.20
3.11
8
<1.00
<1.00
<1.00
<1.00
1.85
1.90
1.00
1.00
2.43
2.45
2.00
2.08
3.20
3.40
3.00
3.08
9
<1.00
<1.00
<1.00
<1.00
1.30
1.30
1.48
1.00
2.53
2.45
2.56
2.53
3.08
3.00
3.04
3.00
10
<1.00
<1.00
<1.00
<1.00
1.30
1.30
1.30
1.00
2.11
2.38
2.00
2.56
3.26
3.18
3.30
3.04
11
<1.00
<1.00
<1.00
<1.00
1.60
1.30
1.48
1.60
2.15
2.30
1.95
1.90
3.15
3.15
3.15
3.08
12
<1.00
<1.00
<1.00
<1.00
1.70
1.85
1.30
1.00
2.28
2.26
2.00
2.00
3.70
3.34
3.40
3.08
13
<1.00
<1.00
<1.00
<1.00
1.60
1.60
1.48
1.30
2.15
2.28
1.78
1.95
2.78
2.90
2.90
2.95
14
<1.00
<1.00
<1.00
<1.00
1.60
1.70
1.70
1.78
2.53
2.08
2.76
2.08
3.49
3.08
3.40
3.23
15
<1.00
<1.00
<1.00
<1.00
1.78
1.78
1.78
1.85
2.11
2.45
2.45
2.30
3.08
2.70
3.41
3.41
RYM= 3M Petrifilm Rapid Yeast and Mold method.
FDA BAM= FDA BAM Chapter 18 method for enumeration of yeasts, molds and mycotoxins.
ISO 21527= ISO 21527:2008 Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration for yeast and molds –Part 2: Colony
count technique in products with water activity less than or equal to 0,95
a
Log
10
yeast and mold CFU/g;
b
A and B= Indicated duplicate test portions;
c
Single Grubbs’ outliner;
d
Double Grubbs’ outliner;
e
Data not included in statistical analysis for this contamination level
f
Laboratory data not included in statistical analysis for all contamination levels
g
FDA BAM and ISO 21257 reference method replicates were enumerated at 5 and 7 days (uninoculated test portions) of incubation at 25
o
C
1
Candidates for 2016 Method of the Year
94