113 / 696
ABBOTT NUTRITION DIVISION
TITLE
PAGE NO.
Determination of Biotin in Infant, Pediatric and Adult
Nutritionals by HPLC and Fluorescence Detection
10 of 11
ss: Sample size in g
D
1
: Unit conversion (from per g to per 100 g sample and from ng to
P
g of biotin)
D
1
= (100g/100g)(1
P
g/1000ng) = 1/10 (
P
g/ng/100g)
D
2
: Conversion from sample to reconstituted finish product
For powder: D
2
= 25 g powder
y
(25 g powder + 200 g water) = 25 g
y
225 g = 1/9
For liquid (2 times dilution by weight): D
2
= 2
For ready to feed (RTF, as is): D
2
= 1
3.
Example: Sample Calculation
a)
Analysis of SRM 1849a (1.0020 g)
Sample peak area = 5.8505, C
i
= 40.21 ng/mL, ss = 1.0020 g
Final result: C
p
= C
i
x 50
y
ss x D
1
x D
2
= 40.21 (ng/mL) x 50 (mL)
y
1.0020 x (1/10) (
P
g/ng/100g)
x (1/9) = 22.29
P
g/100g reconstituted finish product
b)
Analysis of Infant Formula RTF (20.0333 g)
Sample peak area = 1.7494, C
i
= 15.78 ng/mL, ss = 20.0333 g
Final result: C
p
= C
i
x 50
y
ss x D
1
x D
2
= 15.78 (ng/mL) x 50 (mL)
y
20.0333 x (1/10) (
P
g/ng/100g)
x 1 = 3.94
P
g/100g RTF
Bio-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE