AOAC SPIFAN ERP Meeting Book (3-16-16)

AOAC SMPR 2015.004

Standard Method Performance Requirements

(SMPRs) for Total Vitamin B

(Niacin) in Infant and

Adult/ Pediatric Nutritional Formula

Intended Use: Reference Method for Dispute Resolution

1 Applicability

Determinations of total vitamin B

(niacin) in all forms of infant,

adult, and/or pediatric formula (powders, ready-to-feed liquids, and

liquid concentrates). Total B

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(CAS No. 59-67-6) and nicotinamide (CAS No. 99586-65-9), and

reported as niacin.

2 Analytical Technique

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performance requirements is acceptable.

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number of replicate measured quantity values and a reference

quantity value.

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quantitative result.

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repeatability standard deviation (SD

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amide, CAS No. 99586-65-9.

4 Method Performance Requirements

See

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5 System Suitability Tests and/or Analytical Quality Control

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6 Reference Material(s)

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infant/adult nutritional powder prepared by a manufacturer of

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7 Validation Guidance

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8 Maximum Time-to-Result

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Approved by AOAC Stakeholder Panel on Infant Formula and

Adult Nutritionals (SPIFAN). Final Version Date: April 17, 2015.

Effective Date: April 17, 2015.

Table 1. Method performance requirements

Analytical range

200–10000

Limit of quantitation (LOQ)

Recovery

90–110%

Repeatability (RSD

)

Reproducibility (RSD

)

Concentrations apply to: (a) “ready-to-feed” liquids “as is”; (b)

reconstituted powders (25 g into 200 g water); and (c) liquid concentrates

diluted 1:1 by weight.

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