434 / 696
Table 2. Method Performance Requirements for Total Choline (SMPR 2012.013)
Analytical range
2-250
a
Limit of detection (LOD)
0.7
a
Limit of quantitation
2.0
a
Repeatability (RSD
r
)
2
a
20
a
100
a
200
a
Recovery
90 to 110% of mean spiked recovery over the range of the
assay
Reproducibility (RSD
R
)
2
a
20
a
100
a
200
a
a
mg/100 g Concentrations apply to: (1) “ready-to-feed’ liquids “as is”; (2) reconstituted powders (25 g into 200 g water);
and (3) liquid concentrates diluted 1:1 by weight. For all concentrations, choline will be expressed as mg/100 g
reconstituted liquids. Report as total choline.
Table 3. Method Performance Requirements for L-Carnitine (SMPR 2012.010)
Analytical range
0.16-20
b
Limit of quantitation (LOQ)
b
Repeatability (RSD
r
)
0.16-20
b
8%
Recovery
90 to 110% of mean spiked recovery over the range of the
assay
Reproducibility (RSD
R
)
0.16-20
b
a
Concentrations apply to: (1) “ready-to-feed’ liquids “as is”; (2) reconstituted powders (25 g into 200 g water); and (3)
liquid concentrates diluted 1:1 by weight.
b
mg/100 g reconstituted final product.
Chol-08/Carn-07 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE