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SW
1
= weight of material delivered to a volumetric flask (RTF, or reconstituted powder) and diluted as per
sample table guidelines. For example, a RTF product of 0.21-1.0% fructan would have a SW
1
of 7 g (±10%) into
a 200 mL volumetric flask.
SW
2
= weight of solution from volumetric flask per sample table guidelines (volumetric dilution of SW
1
). Value
should be 0.2 g ± 10%.
PW
= weight of powder product weighed to be reconstituted (5 g ± 10%).
WW
= weight of water used to reconstitute powder products (40 g ± 10%).
b.
Dilution factor (DF)
–
Calculated as follows:
Low-level fructan (RTF 0.03-0.2%, powder 0.27-1.0%)
DF = 50 mL
x
2 mL
Mid-level fructan (RTF 0.21-1.0%, powder 1.1-10%)
DF = 200 mL
x
2 mL
High-level fructan (RTF 1.1-5.0%, powder 11-45%)
DF = 500 mL
x
2 mL
Commodity DF (46-100%)
DF = 1000 mL x
2 mL
c.
Commodity factors (CF)
–
As in Table 2-6 (Type determined by qualitative ID methodology in Part 1 if not
known).
Table 2-6
CF’s
Group
Commodity type
CF
1
scFOS and oligofructose (DP= 3.60-4, factor based on DP
Avg
= 4)
1.233
2 H.S.I., Synergy 1 and native inulin (DP = 5.49-10, factor based on DP
Avg
= 6.95) 1.068
3
Enriched Inulin (Avg. DP= 11-50, factor based on DP
Avg
= 20)
0.9526
These factors were derived as follows:
Eqn. #3:
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Fos-04 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE