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AsureQuality Laboratory Services
8. CHROMATOGRAPHIC CONDITIONS
1. Mobile Phase A : 0.1% Phosphoric acid
2. Mobile Phase B : 100% Acetonitrile
3. Mobile Phase C : 80% Acetonitrile
4. Column: Phenomenex Kinetex Phenyl-Hexyl, 00F-4495-E0 (150 x 4.6mm x 2.6μm x 100Å)
5. Column Temperature: 25±2ºC
6. Retention time: 16 to 17 minutes
7. Run time: 27 minutes
8. Detector: PDA operating at 200nm (PDA scan 200 to 350nm)
9. Injection volume: 100μL
Table 2: Gradient Program
Time (min) Flow rate (mL/min) Mobile Phase A (%) Mobile Phase B (%) Mobile Phase C (%)
0.0
0.6
90
10
0
18.0
0.6
90
10
0
18.5
0.8
0
0
100
24.0
0.8
0
0
100
24.5
0.6
90
10
0
27.0
0.6
90
10
0
9. SENSITIVITY
Limit of Quantitation (LOQ) was calculated based on the lowest working standard and the
dilution factor.
LOQ = (1 x 100) / (20 x 50) = 0.1μg/100g (1ppb)
1 = 1μg/100mL lowest standard
100 = Volume (mL)
20 = 20g sample
50 = Volume (mL) loaded on immunoaffinity column
1= Final volume (mL)
Bio-02 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE