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Do you agree that the evidence or data from this and previous studies support the proposed applicability statement?
ER 1
Yes
ER 2
Yes
ER 3
Yes
ER 4
Yes
ER 5
Yes
ER 6
Yes
ER 7
Yes
ER 8
Yes
Are there sufficient data points per product evaluated in accordance with AOAC requirements?
ER 1
Yes
ER 2
Yes
ER 3
Yes
ER 4
Yes
ER 5
Yes
ER 6
Yes
ER 7
Yes
ER 8
Yes
General Comments about the Method Scope/Applicability:
ER 1
The method scope and applicability are clearly described, and the Petrifilm protocols have been clearly
presented. Both incubation temperature options were also clearly stipulated. Description of the look like
colonies were sufficiently explained.
ER 2
A statement about growth curve for the yeast selected was made on page 3, row 14-15. This culture
incubated for 48h while there are strains requiring 5-7 days incubation. The method includes up to 60 h
incubation. Reviewer recommends applicability statement to this point. The background section could
include this point and then the discussion could address it.
ER 3
Almonds and Ground Beef have been used for the collaborative study which are both appropriate
matrices and were used in the pre-collaborative study.
ER 4
No additional comments
ER 5
The method is useful for rapid quantification of spoilage microorganisms
ER 6
Accurately reflects breadth and depth of study
ER 7
None
ER 8
1. Page 5, lines 27-29, Sterile Diluent. The method lists several diluents that can be used with this
method, including 0.1% peptone water. All of the diluents, other than 0.1% peptone water, should be
removed from the method, since the method was validated with 0.1 peptone water and no other diluent.
The implied conclusion is that the method, as given by the draft official method, is that it can be run with
any diluent even though it was only validated with 0.1 % peptone water. 2. Page 5, lines, 7 - 9,
applicability (here and elsewhere in the report). The method refers to yogurt, ready-made-pie, frozen
ground beef patties, sandwiches, and dehydrated soup. These are not specific enough. Yogurt and beef
should have fat percentages. The types of pie, sandwiches and dehydrated soup should be specified. For
example, apple pie, chicken soup, tuna sandwich... The applicability statement is too broad as written.
ERP PROFILE SUMMARIES
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