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Do you agree that the evidence or data from this and previous studies support the proposed applicability statement?
ER 1
Yes
ER 2
Yes
ER 3
Yes
ER 4
Yes
ER 5
No
ER 6
Yes
ER 7
No
ER 8
Yes
Are there sufficient data points per product evaluated in accordance with AOAC requirements?
ER 1
Yes
ER 2
Yes
ER 3
Yes
ER 4
Yes
ER 5
No
ER 6
Yes
ER 7
No
ER 8
General Comments about the Method Scope/Applicability:
ER 1
Method is applicable to a wide variety of foods judging from the PTM study.
ER 2
NA
ER 3
No additional comments
ER 4
Cottage cheese has been used for this collaborative study which is an appropriate matrix since it was also
used in the pre-collaborative study.
ER 5
The method scope and applicability are clearly described, and the various enrichment protocols have
been extensively presented and summarized in tables. The possibility to use different enrichment media,
with one or two enrichment steps according to the matrix, makes the overall analysis more complex, but
all information can be found in the document. Nonetheless, no indication has been provided regarding
the choice of mLRB versus Demi-Fraser for the user: does it matter?
ER 6
Listeria is a bacterium frequently isolated from different types of food and surfaces. This method allows
to detect Listeria in an easy and rapid way with a high sensitivity and specificity.
ER 7
Overall, the method looks good. My main question concerns the application of the guidelines (Appendix
J, section 4.3.4, "Matrix Selection"), which affects applicability. Only Demi-Fraser broth was used in the
collaborative study, but the official method calls for both Demi-Fraser and mLRB. Section 4.3.4 states
"For methods with more than one sample preparation/enrichment, one matrix per procedure may be
required in the collaborative study. The determination if the procedures differ significantly to warrant
expanding the collaborative study is made by the appropriate method volunteer(s) in consultation with
the Study Director." Was a decision reached between the study director and volunteers? If not, then it
seems to me that the method may only be validated for use with Demi-Fraser broth.
ER 8
Appropriate
ERP PROFILE SUMMARIES
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