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Technical Suggestion
•
83‐86: the size of the eukaryotic genomes makes
using a 10X concentration of the genome difficult
as the quantity of DNA is huge. Using a
concentration equivalent to the 10X of the
bacterial genome still provides a background but is
not so viscous. Alternatively, you can place a
maximum DNA concentration on testing such as
100ug of background DNA.
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Proposed Change
•
83‐86:Organisms may be tested as pools
containing ten organisms each represented at 10
times the AMDL. Cell lines should be tested
individually at a DNA concentration equivalent to
10X AMDL. These tests should be negative for the
target sequence. The same pools can be spiked
with 2X the AMDL for the target organism, mixed
and tested. These tests should be positive for the
target in the high DNA background
.
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