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Burkholderia mallei/pseudomallei

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Bacillus anthracis

Inclusivity Panel – Tier 1

A4.1.1

Bacillus anthracis.

If any listed strain is not available, an alternate strain shall be selected

from the same VNTR group. If an alternate strain from the same VNTR group is not available, a

strain from the same genotype shall be selected. If an alternate strain from the same genotype is not

available, a strain different than those listed below shall be selected if available;

Tier 1

Bacillus anthracis

Inclusivity Panel

Species

Strain

Notes

Bacillus anthracis

Ames

pXO1+, pXO2+; VNTR group A3b; genotype 62

Bacillus anthracis

BA1035

pXO1+, pXO2+, VNTR group B1; genotype 82

Bacillus anthracis

BA0018

pXO1+, pXO2+; VNTR group A1a; genotype 7

Bacillus anthracis

K3

pXO1+, pXO2+; VNTR group A3c; genotype 67

Bacillus anthracis

Ohio ACB

pXO1+, pXO2+; VNTR group A3d; genotype 68

Bacillus anthracis

PAK-1

pXO1+, pXO2+; VNTR group A2; genotype 29

Bacillus anthracis

A

Pasteur

pXO1-, pXO2+; VNTR group A1a; genotype 8

Bacillus anthracis

RA3

pXO1+, pXO2+; VNTR group B2; genotype 80

Bacillus anthracis

SK-102

pXO1+, pXO2+; VNTR group A4; genotype 69

Bacillus anthracis

B

Sterne

pXO1+, pXO2-; VNTR group A3b; genotype 59, 61

Bacillus anthracis

Turkey 32

pXO1+, pXO2+; VNTR group A1b; genotype 23

Bacillus anthracis

B

V770-NP-1R

pXO1+, pXO2-; VNTR group A3A; genotype 45

Bacillus anthracis

Vollum 1B

pXO1+, pXO2+; VNTR group A4; genotype 77

Adapted from Official Methods of Analysis of AOAC INTERNATIONAL (2016) 20th Ed., AOAC INTERNATIONAL, Rockville, MD,

USA, AOAC SMPR 2010.003,

www.eoma.aoac.org

,and

Bacillus anthracis

2016.006 - Journal of AOAC International, Volume 99, Number

4, July-August 2016, pp. 1084-1089.

https://doi.org/10.5740/jaoacint.SMPR2016.006.

A

This strain should not be included in the inclusivity panel if the product being tested only utilizes a pXO1 assay, as this strain is expected to

be pXO1 negative.

B

These two strains should not be included in the inclusivity panel if the product being tested only utilizes a pXO2 assay, as these strains are

expected to be pXO2 negative.