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Bacillus anthracis

Inclusivity Panel – Tier 2

A4.2.1

Bacillus anthracis

. If any listed strain is not available, an alternate strain shall be selected

from the same VNTR group. If an alternate strain from the same VNTR group is not available, a

strain from the same genotype shall be selected. If an alternate strain from the same genotype is not

available, a strain different than those listed below shall be selected if available;

Tier 2

Bacillus anthracis

Inclusivity Panel

Species

Strain

Notes

Bacillus anthracis

Turkey 32

pXO1+, pXO2+; VNTR group A1b; genotype 23

Bacillus anthracis

K3

pXO1+, pXO2+; VNTR group A3c; genotype 67

Bacillus anthracis

Ohio ACB

pXO1+, pXO2+; VNTR group A3d; genotype 68

Bacillus anthracis

Ames

pXO1+, pXO2+; VNTR group A3b; genotype 62

Bacillus anthracis

BA1035

pXO1+, pXO2+; VNTR group B1; genotype 82

Bacillus anthracis

BA0018

pXO1+, pXO2+; VNTR group A1a; genotype 7

Bacillus anthracis

PAK-1

pXO1+, pXO2+; VNTR group A2; genotype 29

Bacillus anthracis

RA3

pXO1+, pXO2+; VNTR group B2; genotype 80

Bacillus anthracis

SK-102

pXO1+, pXO2+; VNTR group A4; genotype 69

Bacillus anthracis

A

Sterne

pXO1+, pXO2-; VNTR group A3b; genotype 59, 61

Bacillus anthracis

A

V770-NP-1R

pXO1+, pXO2-; VNTR group A3a; genotype 45

Bacillus anthracis

Vollum 1B

pXO1+, pXO2+; VNTR group A4; genotype 77

Adapted from Official Methods of Analysis of AOAC INTERNATIONAL (2016) 20th Ed., AOAC INTERNATIONAL, Rockville, MD,

USA, AOAC SMPR 2010.003,

www.eoma.aoac.org ,

and

Bacillus anthracis

2016.006 - Journal of AOAC International, Volume 99,

Number 4, July-August 2016, pp. 1084-1089.

A

These two strains should not be included in the inclusivity panel if the product being tested only utilizes a pX02 assay, as these strains

are expected to be pXO2 negative.

Bacillus anthracis

Exclusivity - Tier 1