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the velocity of the deformation- and this mechanical component can
sometimes be lost or ignored in certain experimental setups and tech-
niques. In fact, this viscous response may prove just as enlightening to
cell mechanics as the elastic response more commonly measured alone
until recently. This talk will discuss these important issues that must be
considered when AFM techniques are applied to cells and other biologi-
cal materials.
Speaker
Sophia Hohlbauch, Asylum Research, an Oxford Instruments Company
1:30 pm–3:00 pm
KinTek Corporation
Why You Should Fit Kinetic and Equilibrium Binding Data
Using Kintek Explorer Software
KinTek Explorer
software offers the fastest, most dynamic and robust
method of fitting kinetic or equilibrium binding data. Based on fast
numerical integration of rate equations, data are fit without the often-
inaccurate approximations needed to derive equations. Rather than fit-
ting data to extract “observed rates” or Eigenvalues, which must be then
interpreted in second step,
KinTek Explorer
yield rate and equilibrium
constants directly while accounting for both the rate and amplitude of
observable reactions. By modeling the experiments exactly as per-
formed, all details of the experimental setup are included, eliminating
errors in interpretation. Moreover, multiple experiments can be fit
simultaneously to a single unifying model. Fast dynamic simulation using
proprietary methods for numerical integration allows you to explore
parameter space and learn kinetics. Don’t be fooled by other vendors
pretending to do the same. Only
KinTek Explorer
offers such robust and
dynamic data fitting.
In this presentation, Professor Johnson will introduce the theory and
operation of the software to show you how easy it is to fit data to
any model you care to input. Examples of experiments that can
be fit include: transient and single turnover stopped-flow kinetics,
steady state kinetics, slow onset inhibition, equilibrium titrations, rapid-
quench-flow kinetics, temperature dependence, voltage-dependent
rate constants. In addition time-resolved absorbance or fluorescence
and pH-dependent spectra can be analyzed by singular value decom-
position to yield spectra and time- or pH-dependence of each spe-
cies. In addition to describing
KinTek Explorer’s
basic features, Dr.
Johnson will introduce new features and will be available to help
you to fit your own data. Learn about what you are missing in your
own data fitting. See
www.kintek-corp.comfor more information.
Speaker
Kenneth A Johnson, President, KinTek Corporation; Professor of
Biochemistry, University of Texas at Austin
3:30 pm–5:00 pm
Bruker Nano Surfaces
Advances in Live Super-resolution Imaging Using the Vutara
352 Microscope
Super-resolution microscopy has made a significant impact in the field of
biological imaging by enabling a ten-fold improvement in spatial resolu-
tion over traditional light microscopy techniques. Most of the imaging
has been so far targeted at fixed specimens with a few live cell applica-
tions. The Vutara 352 microscope has been engineered towards live-cell
imaging by enhancing spatial and temporal resolution in single molecule
localization super-resolution. The sCMOS detector in the Vutara 352
enables imaging at 800 fps at full ROI and at video frame rates at reduced
ROI. Two color simultaneous imaging can be applied in both super-res-
olution live cell and 3D particle tracking experiments. The biplane based
detection path enables imaging thicker samples such as whole mount
Drosophila and offers deeper penetration into tissues. The Vutara 352
also includes real time localization along with several statistical and live
cell analysis features for processing data. In summary, the Vutara 352
microscope is a powerful super-resolution imaging and analysis tool.
Speaker
Manasa Gudheti, Applications Scientist at Bruker – Fluorescence
Microscopy Business
5:30 pm–7:00 pm
Sutter Instrument
Scientists Empowering Scientists
Patch clamp electrophysiology has matured from a highly specialized
scientific technique to a recognized method used to address a variety
of experimental questions. Sutter Instrument introduces a highly flex-
ible, intuitive patch clamp instrumentation and software package that
enables the experimenter to quickly set up and perform routine tasks,
yet remains highly configurable to meet the demands of the experienced
electrophysiologist.
We will demonstrate how the IPA™ Integrated Patch Amplifier and
SutterPatch™ software can be used for a variety of commonly performed
assays, including the characterization of an ionic current and the record-
ing of synaptic events in tissue slices. We will also highlight how the IPA
and SutterPatch software provide easy access and flexibility to perform
and fine-tune the most challenging acquisition and analysis scenarios.
Building on the basic pipette pulling tutorials presented at the 2015 user
meeting and a mid-year webinar, we will further teach advanced tech-
niques that enable the user to create specialized pipette morphologies
for unique applications.
There will be plenty of opportunity for discussion with hosts and speak-
ers from the Sutter Instrument Tech Support Team.
Who should attend?
• Electrophysiologists who use amplifiers, micropipettes and micro-
manipulators for patch clamp, sharp electrode or extracellular
recordings.
• Researchers who perform microinjections, including nuclear trans-
fer, sperm injection and application of substances into cell cultures
or intact organisms.
Speakers
Jan Dolzer, Tech Support and Product Development, Sutter Instrument
Gregory Hjelmstad, Tech Support and Product Development, Sutter
Instrument