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Room 505: Tuesday, March 1
11:30 am–1:00 pm
Bruker Nano Surfaces
BioScope Resolve BioAFM – Unrivalled AFM Biomechanics
and Resolution
In this presentation we will introduce new capabilities for cell mechanobi-
ology and highest resolution cell and molecular imaging available on the
BioScope Resolve BioAFM. We will explain how innovations in force con-
trol and instrument design have enabled BioScope Resolve to be the first
AFM to image microvilli on live cells and to consistently resolve the double
helix of DNA, while on the inverted microscope. To enable highest resolu-
tion and accurate cell mechanics data, the entire AFMmechanical loop of
BioScope Resolve has been designed for stability, specifically on biologi-
cal samples, with biological sample carriers, and when mounted on the
inverted microscope. Designed for Bruker’s exclusive PeakForce Tapping,
BioScope Resolve eliminates the need for fluid cantilever tuning entirely
with ScanAsyst and provides quantifiable pN force control for imaging and
force mapping. The combination of PeakForce Tapping and FASTForce
Volume provides the broadest range of frequencies for mechanical
characterization, with a new no-touch calibration method guarantee-
ing accurate calibration. As we will show in the presentation, BioScope
Resolve also features synchronization of these unique mechanical mea-
surements with fluorescence, enabling new kinds of correlative studies.
Speaker
Marcin Walkiewicz, PhD, Applications Scientist, Bruker – Atomic Force
Microscopy Business
Room 513: Sunday, February 28
10:30 am–12:00 pm
Carl Zeiss Microscopy LLC
Technology Innovations: ZEISS LSM 880 Confocal with
Airyscan and ZEISS Lightsheet Z.1
These microscopes from ZEISS address both ends of the spectrum of sam-
ples, live high speed imaging with superresolution and high speed imaging
of large live and fixed tissues. Learn how ZEISS LSM 880 with Airyscan
maintains the mantra that each photon of emission light is precious, while
expanding the triangle of sensitivity, resolution and speed of acquisition.
ZEISS LSM 880 with Airyscan allows you to use multicolor samples with
any label and get image quality like you have never seen before. With
Airyscan you are always able to select the optimal acquisition strategy for
your sample: Simply decide whether you want to gain 1.7x higher resolu-
tion in all three dimensions – resulting in a 5x smaller confocal volume.
Or push the sensitivity beyond the limits of all conventional confocal
microscopes; or use the increase in signal-to-noise ratio to speed up your
image acquisition.
Traditionally, deeply imaging into intact tissue typically requires multi-
photon excitation to penetrate deeper than near the surface of a tis-
sue. Using a “clearing” method to remove the light obstructing opaque
molecules from a tissue has been another technique for deep imaging.
Techniques such as SCALE, CLARITY, ClearT, SeeDB, CUBIC and others
have allowed researchers to image deeper than a millimeter into cleared
animal model brains and organs.
ZEISS Lightsheet Z.1 features high speed image acquisition and greatly
reduced photo damage making imaging of live developmental samples
and fixed and cleared tissues easier than ever before. Come learn about
using the innovative ZEISS Lightsheet Z.1 microscope for imaging of fixed
and cleared tissues.
Speaker
Joseph Huff, Product Marking Manager, Laser Scanning and
Superresolution Microscopy, Carl Zeiss Microscopy LLC
Scott Olenych, Product Marketing Manager, Imaging Products, Carl Zeiss
Microscopy LLC