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H
ALL
& K
EULER
: J
OURNAL OF
AOAC I
NTERNATIONAL
V
OL
. 92, N
O
. 1, 2009
51
Table 1. Effect of delayed reading on sample absorbance values and percentage change in absorbance relative to
no time delay
a
Time delay to reading, min
Incubation condition
Glucose, g/mL
0
15
30
45
60
Standard solution:GOPODk reagent (0.5:2.5)
Absorbance at 505 nm
35 C for 45 min
0
0.000
0.001
0.001
0.002
0.003
40
0.239
0.238
0.237
0.234
0.236
60
0.359
0.357
0.354
0.352
0.351
100
0.594
0.586
0.582
0.579
0.579
(Absorbance/0 time delay absorbance) 100
40
99.7%
99.1%
98.1%
98.8%
60
99.2%
98.6%
97.9%
97.8%
100
98.7%
98.0%
97.5%
97.5%
Absorbance at 505 nm
50 C for 20 min
0
0.000
0.001
0.003
0.003
0.003
40
0.238
0.240
0.242
0.237
0.235
60
0.358
0.357
0.356
0.353
0.351
100
0.589
0.582
0.580
0.577
0.570
(Absorbance/0 time delay absorbance) 100
40
100.5% 101.4%
99.6%
98.7%
60
99.9%
99.4%
98.7%
98.0%
100
98.8%
98.6%
98.0%
96.9%
Time delay to reading, min
0
10
20
30
40
Standard solution:GOPODk reagent (0.1:3.0)
Absorbance at 505 nm
50 C for 20 min
0
0.000
0.002
0.004
0.000
0.001
400
0.455
0.453
0.452
0.445
0.444
600
0.674
0.671
0.671
0.662
0.656
1000
1.114
1.107
1.098
1.087
1.079
(Absorbance/0 time delay absorbance) 100
400
99.6%
99.3%
97.8%
97.5%
600
99.5%
99.5%
98.1%
97.3%
1000
99.4%
98.6%
97.6%
96.9%
a
Values presented as least-squares means and calculated percentages.