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Abbott Nutrition Division
Simultaneous Determination of Total Vitamin B
6,
B
2,
B
3
and B
1
in
Infant Formula Products by LC-MS/MS Using Enzymatic Digestion
A.
SAFETY
LABORATORY CHEMICALS SHOULD BE HANDLED WITH THE PRECAUTIONS, CARE
AND RESPECT THAT ALL POTENTIALLY HAZARDOUS MATERIALS REQUIRE. AVOID
SKIN CONTACT AND INJESTION OF ALL COMPOUNDS, REAGENTS AND SOLUTIONS
USED IN THIS PROCEDURE.
MOST REAGENTS USED IN THIS METHOD POSE MINIMAL HEALTH AND/OR FIRE
HAZARDS. THOROUGHLY FLUSH ANY AREA COMING IN CONTACT WITH ANY
REAGENTS USED IN THIS METHOD WITH WATER.
IN GENERAL, DILUTE SOLUTIONS PRODUCED TO SUPPORT THE PREPARATION OF
SAMPLES FOR ANALYSIS, MOBILE PHASE COMPONENTS AND SAMPLE EXCESS
SAMPLE EXTRACT VOLUMES CAN BE DISCARDED DOWN THE DRAIN. WASTES
GENERATED SHOULD BE DISCARDED IN ACCORD WITH ALL CORPORATE, LOCAL
AND FEDERAL REGULATIONS.
THROUGHOUT THIS DOCUMENT STEPS REQUIRING SPECIFIC SAFETY PRECAUTIONS
OR PERSONAL PROTECTIVE EQUIPMENT ARE DENOTED BY THE FOLLOWING
SYMBOLS:
USE OF GLOVES IS REQUIRED. LATEX, NITRILE OR RUBBER GLOVES ARE
RECOMMENDED.
PERFORM THIS PROCEDURAL STEP USING A WORKING FUME HOOD. THE
CHEMICALS USED IN THIS STEP REPRESENT AN INHALATION HAZARD.
1.
METHANOL
TOXIC!
CAN CAUSE BLINDNESS. CUMULATIVE POISON. HARMFUL IF
INHALED, SWALLOWED, OR ABSORBED THROUGH SKIN. USE WITH ADEQUATE
VENTILATION AND AVOID BREATHING VAPORS. AVOID CONTACT WITH SKIN OR
EYES.
19
BVIT-02
FOR ERP/WORKING GROUP USE ONLY
DO NOT DISTRIBUTE