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Abbott Nutrition Division
Simultaneous Determination of Total Vitamin B
6,
B
2,
B
3
and B
1
in Infant
Formula Products by LC-MS/MS Using Enzymatic Digestion
dipegyl, dipigyl, delonin amide, endobion, factor pp, hansamid, inovitan pp, nandervit-n, niacevit,
niacinamide, niamide, nicamina, nicamindon, nicasir, nicobion, nicofort, nicogen and nicomidol.
The chemical structure for niacinamide is shown below:
Niacinamide may be partially hydrolyzed to nicotinic acid in the predrying evaporation step
during processing of Similac powder (nicotinic acid may also be determined with this method in
certain products).
The molecular formula of nicotinic acid is C
6
H
5
N0
2
and its molecular weight is 123.11 g/mol.
The CAS# for nicotinic acid is 59-67-6.
The chemical structure for nicotinic acid is shown below:
NOTE: USP Catalog 1461003, niacin, is nicotinic acid.
USP catalog 1462006, niacinamide is the compound fortified into Abbott Nutrition
products and the primary form of niacin quantified by this method.
C.
APPARATUS
1.
General Laboratory Equipment/Facilities
a)
Analytical Balances: Capable of accurately weighing at least 5 mg (for standards), an Analytical
four place, and a top loading two place capable of weighing to several hundred grams.
Examples: Mettler Model AE163, Mettler Model AE100 and Mettler Model PG2002, or
equivalent.
b)
Laboratory Water Purifier: Millipore Milli-Q Water Purification System, or equivalent.
N
NH
2
O
N
OH
O
25
BVIT-02
FOR ERP/WORKING GROUP USE ONLY
DO NOT DISTRIBUTE