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Abbott Nutrition Division
Simultaneous Determination of Total Vitamin B
6,
B
2,
B
3
and B
1
in Infant
Formula Products by LC-MS/MS Using Enzymatic Digestion
c)
Bottle-Top Dispenser: Capable of dispensing volumes of 25.0mL (
±
1mL).
Example: Brand Tech Dispensette III, or equivalent.
d)
Vortex Mixer: Vortex-Genie, or equivalent.
e)
Laboratory Refrigerator
f)
Laboratory Freezer
g)
Magnetic Stir Plate: Variomag multipoint electronic magnetic stir-plate, or equivalent.
h)
Room Light Shields: A.L.P. Protect-A-Lamp, UV cutoff at 460 nm.
i)
Volumetric Flasks: Various sizes including 5-mL, 10-mL 25-mL, 50-mL, 100-mL, 250-mL, 500-
mL, 1000-mL and 2000-mL.
j)
Disposable Pasteur Pipettes
k)
Amber Bottles: Volume capacity of 50 or 100 mL.
l)
Self-Standing Polypropylene Tubes: Cryovials; 5-mL and 10-mL volume capacity.
m)
Weighing Vessels: Various including disposable weighing boats and glass weighing funnels.
n)
Positive Displacement Pipettes: Gilson Microman 10, 25, 100, 250, and 1000 µL.
o)
Positive Displacement Pipette Tips: Gilson Capillary Piston 10, 25, 100, 250, and 1000 µL.
p)
Plastic Syringes. B&D 3-mL, or equivalent.
q)
Syringe Filters: PTFE 0.20µm syringe filters, Acrodisc 25-mm, or equivalent.
r)
50-mL Self-Standing Transport Tubes: Superior Scientific, Ltd., or equivalent.
s)
Autosampler Vials: Waters autosampler vials; 9-mm amber with screw top 12x32 mm pre-split
PTFE-silicon septa; Waters Part # 186000847C.
2.
Instrumentation
NOTE: The following instrumentation has been specifically demonstrated as adequate
for the implementation of this method. Comparable instrumentation may be
substituted with sufficient qualification.
a)
Liquid Chromatograph (LC): Waters Acquity Ultra Performance Liquid Chromatograph (UPLC).
b)
Chromatographic Columns: 2.1 x 50 mm, 1.7-µm Waters BEH C-18Column, and 2.1 x 5mm
Vanguard Column.
c)
Prefilter for Waters Acquity UPLC Column.
d)
Waters Acquity UPLC 10µL Sample Loop.
t)
Tamdem Quadrupole Mass Spectrometer (MS/MS) equipped with an Electrospray Ionization
(ESI) Source: Waters Quattro Premier XE or equivalent.
e)
IBM Personal Computer or equivalent. Suitable for operation of the chromatography data system
software specified below.
26
BVIT-02
FOR ERP/WORKING GROUP USE ONLY
DO NOT DISTRIBUTE