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Abbott Nutrition Division
Simultaneous Determination of Total Vitamin B
6,
B
2,
B
3
and B
1
in Infant
Formula Products by LC-MS/MS Using Enzymatic Digestion
b)
Mass Spectrometer instrument configuration and operating parameters are provided below.
Table 2. Mass spectrometer configuration and operation parameters
Instrument
Quattro Premier XE
Ionization Mode
ESI+ (Electrospray; Positive Ion)
Capillary Voltage
3.50
Cone Voltage
Various
Extractor (V)
5.0
RF Lens (V)
0.0
Source Temperature
120 ºC
Desolvation Temperature
325 ºC
Cone Gas Flow
50 L/Hr
Desolvation Gas Flow
800 L/Hr
LM 1 (Low Mass) Resolution
10
LM 2 (Low Mass) Resolution
10
Ion Energy 1
0.2
Entrance Lens
1
Collision Energy
Various
Exit Lens
1
HM 1 (High Mass) Resolution
10
HM 2 (High Mass) Resolution
10
Ion Energy 2
1.0
Multiplier
710
Collision Cell Pressure
~4 e-3
These parameters are guidelines for the operation of the Quattro Premier XE. These
parameters may be changed at the discretion of a qualified LC-MS/MS scientist and the
approval of the method supervisor based on the instrument performance provided
all system
suitability and control sample parameters are met.
28
BVIT-02
FOR ERP/WORKING GROUP USE ONLY
DO NOT DISTRIBUTE