AOAC SPIFAN Review Team Meeting Book-July 22, 2015

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Simultaneous Determination of Thiamine HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide),
Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant
Formula and Adult Nutritionals
CONFIDENTIAL METHOD FOR ERP ASSESSMENT ONLY
PAGE 3 OF 13
98%)
(C8D3H9N2O2.2HCl)
contamination
16 Isotope Biotin (chemical
purity 97%) (6,6-D2,
98%)
(C10D2H14N2O3S)
97% N/A CIL Until
exhausted or
evidence of
contamination
Room temp
away from
light and
moisture
17 Thiamine Hydrochloride
(Vitamin B1 )
(C12H17ClN4OS-HCl)
99.8% N/A USP See remark
below
2-8 C
18 Riboflavin (Vitamin B2)
(C17H2ON4O6)
100 % N/A USP See remark
below
2-8 C
19 Nicotinamide
(C6H6N2O), USP
99.9% N/A USP See remark
below
2-8 C
20 Nicotinic acid
(C6H5NO2)
99.8% N/A USP See remark
below
2-8 C
21 Calcium pantothenate
(C18H32CaN2O10)
99% N/A USP See remark
below
2-8 C
22 Pyridoxine hydrochloride
(C8H11NO3. HCl)
99.5% N/A USP See remark
below
2-8 C
23 Pyridoxal hydrochloride
(C8H9NO3.HCl)
≥ 99 % N/A Sigma-
Aldrich
as per COA -20 to-30 C
24 Pyridoxamine
dihidrochloride
(C8H12N2O2. 2HCl)
≥ 98 % N/A Sigma-
Aldrich
as per COA -20 to-30 C
25 Biotin (C10H16N2O3S) 99.3% N/A USP See remark
below
2-8 C
26 Deionized water (DI)
Resistivity ≥ 18.2 M
Fresh
27 75 mM Formic acid-20%
MeOH mixture solution
1 1 year Room
temperature
28 1 N HCl 2 1 year Room
temperature
29 50 mM HCl 3 1 year Room
temperature
30 1 % Formic acid 4 1 year Room
temperature
31 Thiamine Cl (Vitamin
B1) stock internal
standard solution
20 mcg/ml 5 Until
exhausted or
evidence of
contamination
2-8 C
32 Riboflavin (Vitamin B2)
stock internal standard
solution
20 mcg/ml 6 Until
exhausted or
evidence of
contamination
2-8 C
33 Nicotinamide stock
internal standard
solution
270 mcg/ml 7 Until
exhausted or
evidence of
contamination
2-8 C
34 Nicotinic acid stock
internal standard
solution
25 mcg/ml 8 Until
exhausted or
evidence of
contamination
2-8 C
35 Pantothenic acid stock
internal standard
30 mcg/ml 9 Until
exhausted or
2-8 C
B-VIT-01
FOR ERP/WORKING GROUP USE ONLY
DO NOT DISTRIBUTE
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