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Simultaneous Determination of Thiamine HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide),
Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant
Formula and Adult Nutritionals
CONFIDENTIAL METHOD FOR ERP ASSESSMENT ONLY
PAGE 4 OF 13
solution
evidence of
contamination
36
Pyridoxine stock internal
standard solution
32 mcg/ml
10
Until
exhausted or
evidence of
contamination
2-8
C
37
Pyridoxal hydrochloride
stock internal standard
solution
25 mcg/ml
11
Until
exhausted or
evidence of
contamination
2-8
C
38
Pyridoxamine
dihydrochloride stock
internal standard
solution
20 mcg/ml
12
Until
exhausted or
evidence of
contamination
2-8
C
39
Biotin stock internal
standard solution
24 mcg/ml
13
Until
exhausted or
evidence of
contamination
2-8
C
40
Mixed intermediate
internal standard
solution I
14
Until
exhausted or
evidence of
contamination
2-8
C
41
Thiamine HCl stock
standard solution
200 mcg/ml
15
3months
2-8
C
42
Riboflavin stock
standard solution
50 mcg/ml
16
3months
2-8
C
43
Nicotinamide stock
standard solution
1000 mcg/ml
17
3months
2-8
C
44
Nicotinic acid stock
standard solution
100 mcg/ml
18
3months
2-8
C
45
Pantothenic acid stock
standard solution
500 mcg/ml
19
3months
2-8
C
46
Pyridoxine stock
standard solution
174 mcg/ml
20
3months
2-8
C
47
Pyridoxal stock standard
solution
100 mcg/ml
21
3months
2-8
C
48
Pyridoxamine stock
standard solution
73 mcg/ml
22
3months
2-8
C
49
Biotin stock standard
solution
100 mcg/ml
23
3months
2-8
C
50
Mixed water-soluble
vitamin intermediate
standard solution I
24
3months
2-8
C
51
Mixed water-soluble
vitamin intermediate
standard solution II
25
1month
2-8
C
52
Mixed water-soluble
vitamin intermediate
standard solution III
26
1month
2-8
C
53
Mixed water-soluble
vitamin working standard
solution 1-16
27
Freshly
prepared
54
Mobile Phase A: 0.04%
HFBA-0.05% Formic
28
2 weeks
Room
temperature
B-VIT-01
FOR ERP/WORKING GROUP USE ONLY
DO NOT DISTRIBUTE
Page 4