SPADA

Virus Characterization

“Genomic sequencing and limited study of

Variola

virus

surface proteins derived from geographically

dispersed specimens is an essential foundation for

important future work. Such research could be

carried out now, and could require a delay in the

destruction of known stocks, but would not

necessitate their indefinite retention.*”

* Assessment of Future Scientific Needs for Live

Variola Virus

; N.A.Press (1999)

Maximum Likelihood Analysis of Single

Nucleotide Matrices (

Variola virus

)

NON-

WEST

isolates passaged

2-3 times to

high titer master

African

minor

AND

AFRICA

BANGLADESH

NEPAL

INDIA

SUMATRA

MIDDLE

EAST

see

stocks

DNA extracted

Sequenced using

primer walking

methodology

Asian

Variola

major

African

major

EAST ASIA

ORIENT

W AFRICA

BRAZIL

Li Y.

et.al

. PNAS 2007;104:15787-15792 Esposito et. al Science 2006

Alastrim minor