55 / 194
Complementary Set:
Inclusivity Panel
Species
Strain
Sample
Variola virus
102
Crude
Variola virus
103
Crude
Variola virus
66-39
Crude
Variola virus
7124
Crude
Variola virus
7125
Crude
Variola virus
72-119
Crude
Variola virus
73-175
Crude
Variola virus
77-1605
Crude
Variola virus
Bombay
Crust
Variola virus
Brazil-Garcia
Crude
Variola virus
Congo
Crude
Exclusivity Panel - near neighbor (
Orthopoxviruses
)
Species
Strain
Ectromelia virus
Moscow
Monkeypox virus
79-0266
Monkeypox virus
79-0005
Camelpoxvirus
LLC
Camelpoxvirus
V78-I-903
Variola virus
Eth-17
Crude
Variola virus
Harper
Crude
Variola virus
Harvey
Crude
Variola virus
Heidelberg
Crude
Variola virus
Higgins
Crude
Variola virus
Hinton
Crude
Variola virus
Horn
Crude
Variola virus
Horn
Pure
Variola virus
K1629
Crude
Variola virus
Kali Mathu
Crude
Variola virus
Kembula
Crude
Variola virus
Minnesota 124
Crude
Variola virus
MS Lee
Crude
Cowpoxvirus
Brighton
Taterapoxvirus
(Gerbilpox)
Vaccinia virus
Lister
Vaccinia virus
VTH
Vaccinia virus
Wyeth
Vaccinia virus
WYH pGS62-9-v1-1-1
Vaccinia virus
Rabbitpoxvirus
Skunkpovirus
Exclusivity Panel - other rash-causing illnesses
Species
Strain
V i ll Z t Vi
W b t
Variola virus
Nepal
Pure
Variola virus
New Dehli
Crude
Variola virus
Nigeria Kuclano
Crust
Variola virus
Nur Islam
Crude
Variola virus
Rumbec
Crude
Variola virus
Shahzamon
Crude
Variola virus
Solaiman
Crude
Variola virus
Stillwell
Crude
Variola virus
V68-59
Crude
Variola virus
V70-222
Crude
Variola virus
V70-228
Crude
Variola virus
Variolator-4
Crude
Variola virus
Yamada
Crude
ar ce a os er rus
e s er
Herpes Simplex Virus-1
HFEM
Rickettsia
conorii
Exclusivity Panel - other negatives
Species
Strain
Myxoma
Human tissue culture cells
Sup-T
Monkey kidney tissue culture cells
BSC-40
Validated diagnostic real-
time PCR assays
Variola virus
:
Diagnostic Creation
and Validation
Target multiple regions of the
genome
Target sequences specific to
Variola virus
Cowpox virus
sequences
acquired after validation
Exhibit extensive
phylogenetic diversity
Contain certain regions
previously thought to be
specific to
Variola virus
• Now assays predicted to
cross-react with
Cowpox virus
D. Carroll, et. al
PLoS One. 2011;6(8):e23086