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Sensitivity analysis:
Two low dilutions (100 and 10 fg/
L) of DNA
Inclusivity panel –
Variola virus
: ≥ 2 purified viral stocks
(at least one from each primary clade)
Current Recommendations:
Tailor Panel to Assay Based on Bioinformatics
(encompass differences in target region)
Specificity analysis:
One high dilution (100 pg/
l) of nucleic acid
Exclusivity panel – near neighbor (
Orthopoxvirus
):
Eurasian: 1
Ectromelia virus
2
Monkeypox viruses
(one from each clade)
1
Camelpox virus
5
Cowpox viruses
(one from each predicted clade)
1
Taterapox virus
4
Vaccinia viruses
(one from each clade)
North American: 1
Raccoonpox virus
1
Skunkpox virus
1
Volepox virus
Specificity analysis (cont.):
One high dilution (100 pg/
l) of DNA
Current Recommendations:
Tailor Panel to Assay Based on Bioinformatics
Exclusivity panel (cont.) – other rash-causing illnesses:
2
Varicella Zoster viruses
(J clade and E1 clade)
2
Herpes simplex viruses
(type 1 and 2)
2 Rickettsia strains
Chordopoxviruses
:
1
Parapoxvirus
(
Orf
)
Negatives:
13 bacteria strains (skin flora)
water