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10
S
alvati
et al
.:
J
ournal of
AOAC I
nternational
V
ol
.
99, N
o
.
3, 2016
Conclusions
This enzymatic digestion LC-MS/MS method provides
simultaneous quantitation of vitamins B
1
, B
2
, B
3
, and B
6
;
and was given First Action status for vitamins B
1
, B
2
, and
B
6
. Method performance was demonstrated over 6 days in 14
different matrixes with three analysts and on two instruments.
Intermediate precision averaged 3.9% and over-spike recovery
was generally 95–105% for all four vitamins.
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