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Reddy:
J
ournal of
AOAC I
nternational
V
ol.
98, N
o
. 4, 2015
1139
K. Example Calibration Curve
See
Figure
2015.04F
.
References
(1)
J. Chromatogr. A
881
, 365(2000)
(2)
J. Chromatogr. A
1139
, 271(2007)
(3) AOAC SMPR
2015.001
J. AOAC Int.
(future issue)
min
0.50 1
.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00
%
0
100
MRMof2 channels,ES-
216 > 186
03121513 Smooth(Mn,2x2)
QC2
3.434e+005
MFA-2NPH_IS
4.04
min
%
0
100
MRMof2 channels,ES-
212 > 182
03121513 Smooth(Mn,2x2)
QC2
9.197e+004
MFA-2NPH
4.05
3.16
2.83 3.35
4.73
6.59
4.81
6.27
5.48 5.88
6.65
Figure 2015.04C. QC overspike Low at 25 ng/g powder.
min
0.50 1
.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00
%
0
100
MRMof2 channels,ES-
216 > 186
03121519 Smooth(Mn,2x2)
QC4
2.967e+005
MFA-2NPH_IS
4.04
min
%
0
100
MRMof2 channels,ES-
212 > 182
03121519 Smooth(Mn,2x2)
QC4
1.622e+006
MFA-2NPH
4.05
Figure 2015.04E. QC overspike High at 500 ng/g
powder.
II.
EXAMPLE CALIBRATION CURVE SEE FIGURE XX
Compound name:MFA-2NPH
Correlation coefficient: r = 0.999397, r^2 = 0.998795
Calibration curve:0.953568 * x+ 0.0208565
Response type: Internal Std ( Ref2 ),Area * ( IS Conc. / IS Area )
Curve type:Linear,Origin:Exclude,Weighting:1/x,Axis trans:None
Conc
-0
10
20
30
40
50
60
70
80
90 100
Response
-0.0
50.0
Conc
Residual
-5.0
0.0
5.0
Figure 2015.04F. Calibration curve and residual plot.
min
0.50 1
.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00
%
0
100
MRMof2 channels,ES-
216 > 186
03121516 Smooth(Mn,2x2)
QC3
3.304e+005
MFA-2NPH_IS
4.04
min
%
0
100
MRMof2 channels,ES-
212 > 182
03121516 Smooth(Mn,2x2)
QC3
3.995e+005
MFA-2NPH
4.05
4.81
6.58
6.27
Figure 2015.04D. QC overspike Med at 100 ng/g powder.
258