AOACSPIFANMethods-2017Awards

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Lin et al.: Journal of AOAC International V ol. 100, No. 1, 2017 151
Table 5. Accuracy: recovery of SRM 1849a
SRM
product Code
Reference
value, mg/kg
powder
Measured value,
mg/kg powder
Recovery versus
CoA, %a
1849a CLC-10b 1.99 1.969 99
2.067 104
2.081 105
2.023 102
2.047 103
1.971 99
2.013 101
1.987 100
1.995 100
aCoA = Certificate of Analysis.
Table 6. Representative chromatograms of tested SPIFAN matrices
Matrix SPIFAN Code Chromatogram
Child Formula Powder, placebo 00847RF00
SRM 1849a CLC10-b
Full-time range to include the riboflavin peak (see below)
Child Formula Powder 00866RF00
Specificity.— Chromatograms from all of the SPIFAN
matrixes helped to establish the specificity of this method for
biotin. See Table 6 for representative chromatograms of tested
SPIFAN matrixes.
Ruggedness .— Several parameters were varied during
validation to establish method ruggedness. Samples were
prepared by three analysts and analyzed with C18 columns from
four different lots. Fresh mobile phase, postcolumn reagents,
intermediate standards, and working standards were made daily
and used during validation.
Conclusions
The data presented in this paper were submitted to the AOAC
Expert Review Panel (ERP) for review at the AOAC Mid-Year
Meeting held on March 16, 2016. The ERP determined that the
data presented here were in accordance with SMPR 2014.005
(5) approved by SPIFAN, and the method was granted First
Action status.
References
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(2) Thompson, L.B., Schmitz, D.J., & Pan, S.-J. (2006) J. AOAC
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(3) Lahély, S., Ndaw, S., Arella, F., & Hasselmann, C. (1999) Food
Chem. 65, 253–258. doi:10.1016/S0308-8146(98)00185-X
(4) Höller, U., Wachter, F., Wehrli, C., & Fizet, C. (2006)
J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 831, 8–16.
doi:10.1016/j.jchromb.2005.11.021
(5) Official Methods of Analysis (2016) 20th Ed., AOAC
INTERNATIONAL, Rockville, MD, SMPR 2014.005.
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