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1394

Jaudzems:

J

ournal of

AOAC I

nternational

V

ol.

98, N

o.

5, 2015

The analytical range for SPIFAN samples was found to be

between 1.4 and 1060 mg/100 g reconstituted product or RTF.

The LOQ was estimated as 1.4 mg/100 g. Results of precision

studies for chloride expressed on reconstituted products are

presented in Table

2015.08A

. RSD

r

ranged from 0.03 to 1.60%

in the range from 20 to 167 mg/100 g RTF, and RSD

R

ranged

from 0.09 to 2.77% over the same range, thus fulfilling the

≤ 2 and 4% performance requirements, respectively. All samples

met the intermediate reproducibility (RSD

R

) performance

requirement except for one RTF sample with high fat content,

which gave a precision value of 10.09%. This RTF sample was

problematic due to heterogeneity and separation since it was

being used beyond its recommended shelf life.

Accuracy was proven by analyzing SRM 1849a in duplicate

on 6 different days and comparing the overall mean to the SRM

informational value (not certified; Table

2015.08B

). The overall

mean was 68.5 mg/100 g for the reconstituted product with

an RSD

R

of 1.59%, equivalent to a recovery of 97.2%. Spike

recovery was performed on 15 different SPIFAN products

(powder and liquid infant formula and adult nutritionals). The

reconstituted powders and RTF products were spiked at two

different levels (50 and 100% of native chloride content) and

analyzed in duplicate on 6 different days (Table

2015.08B

).

Spike recovery (99–104%) was within the tolerance (95–105%)

defined in the SMPR.

All results compared to SMPR values are shown in

Table 

2015.08C

. All results were within the target values

defined in AOAC SMPR 2014.015.

Conclusions

The data presented in this paper were submitted to the AOAC

ERP for review at the AOAC INTERNATIONAL Mid-Year

Meeting held on March 17, 2015. The ERP determined that

the data presented were in accordance with SMPR 2014.015

approved by SPIFAN, and the method was granted First Action

status.

References

(1) AOAC SMPR 2014.015,

J. AOAC Int . 98 , 1079. http://dx.doi.

org/10.5740/jaoacint.SMPR2014.016

(2) Sullivan, D. (2012)

J. AOAC Int

.

95

, 278–297.

http://dx.doi.org/

10.5740/jaoacint.Sullivan_Intro

(3)

Official Methods of Analysis

(2012) 19th Ed., AOAC

INTERNATIONAL, Rockville, MD, Appendix L: AOAC

Recommended Guidelines for Stakeholder Panel on Infant

Formula and Adult Nutritionals (SPIFAN) Single-Laboratory

Validation,

http://www.eoma.aoac.org

90