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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
1
Expert Review Panel for Infant Formula and Adult Nutrition
Evaluation of Method FOS-01
Title:
SPIFAN FOS-01 Single Lab Validation (SLV) for fructan in Infant Formula and Adult Nutritionals
Author:
Steegmans (Beneo-Orafti)
Summary of Method:
Samples are first defatted with hexane. Subsequently, the fructans are extracted from the sample with
boiling water. An aliquot of the extract is hydrolyzed with inulinase. The concentrations of glucose,
fructose and sucrose are chromatographically determined by gaschromatography: an internal standard is
added to the sample followed by drying under vacuum. After treatment of the dried sample with
hydroxylamine, the sugars are transformed to derivatives with N-(Trimethylsilyl)imidazole (TMSI). After
addition of water the volatile derivatives are extracted with iso-octane and this phase is injected on a GC
column. Fructan content is calculated from fructose, corrected for free fructose and fructose released from
sucrose.
Method Scope/Applicability:
The method is applicable for Infant formula, adult / pediatric nutritional formula, and commodity
ingredients.
General comments about the method:
•
The method used is AOAC 997.08 with an adaptation according to Stöber (the incubation with
amyloglucosidase is skipped).
Method Clarity:
•
In general clearly written
•
For the calculation of the fructan content, DP is an important factor. It is unclear how to calculate the
fructan content, when the DP is unknown.
Pros/Strengths:
•
Widely available equipment is used
•
No correction needed for missing part of Fm chains
Cons/Weaknesses
•
Elaborate sample preparation
•
DP needs to be known in order to calculate fructan content
•
Less precise when sucrose content exceeds about three times the total fructan content (according to
Stöber)
Supporting Data
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FOS-01 Review Forms