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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
1
Expert Review Panel for Infant Formula and Adult Nutrition
Evaluation of Method FOS-01
Title: AOAC Official Method 997.08 Fructans in Foods
Single Lab validation for fructan in Infant Formula and Adult Nutritionals.
Author:
Beneo-Orafti – Central Department Research
Summary of Method:
The method is based on AOAC Method 997.08 with some modifications, the chromatographic analyses
were performed by Gas Chromatography.
Based on Stober method, the incubation with amyloglucosidase (AG) is skipped as the performance of the
method is not influenced. Calculations are performed accordingly Strober at all. The samples contain fat are
first defatted by use of hexane.
The fructans are extracted from the sample with boiling water. An aliquot of the extract is hydrolyzed with
inulinase. The concentrations of glucose, fructose and sucrose are chromatographically determined by
gaschromatography: an internal standard is added to the sample followed by drying under vacuum. After
treatment of the dried sample with hydroxylamine, the sugars are transformed to derivatives with N-
(Trimethylsilyl) imidazole (TMSI). After addition of water the volatile derivatives are extracted with iso-
octane and this phase is injected on a capillary column.
Method Scope/Applicability:
Applicable to the determination of added fructans in infant formula and adult nutritionals.
General comments about the method:
Depending on the matrix of the sample, is possible to perform different chromatographic technique as GC
or HPLC.
The samples analized were SPIFAN infant formula an adult nutritionals.
The pure products Orafti HP (inulin), Orafti P95 (FOS) and Nutraflora P95 (FOS) are part of the test
materials kit and were screened in duplicate by 2 different chromatographic techniques, being GC and
HPLC.
Method Clarity:
The method description is clear and easy to follow.
Pros/Strengths:
The determination of the sugars can be done accurately without interferences.
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