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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
1
Expert Review Panel for Infant Formula and Adult Nutrition
Evaluation of Method:
FOS-01
Title:
Fructan in Infant Formula and Adult Nutritionals.
Author:
Monique Steegmans, Beneo
Summary of Method:
Based upon AOAC 997.08 except method incubation with amyloglucosidase (AG) is skipped and the
chromatographic analyses were performed by gas chromatography.
The concentrations of glucose, fructose and sucrose are chromatographically determined by gas
chromatography: an internal standard is added to the sample followed by drying under vacuum.
The samples are first defatted by use of hexane. The fructans are extracted from the sample with boiling
water. An aliquot of the extract is hydrolyzed with inulinase. After treatment of the dried sample with
hydroxylamine, the sugars are transformed to derivatives with N-(Trimethylsilyl)imidazole (TMSI). After
addition of water the volatile derivatives are extracted with iso-octane and this phase is injected on a
capillary column.
Method Scope/Applicability:
Infant Formula and Adult/Pediatric Formulas with fructan % of 0.03g to 5g/100g
General comments about the method:
A comparatively simple approach for analysis of fructans
Method Clarity:
Not the easiest method to follow and method would need to be written in clearer, concise language.
Method Safety Concerns:
Standard laboratory safety, however uses pyridine which can be concern for some labs.
Pros/Strengths:
Relative simple assay and GC-FID are found in many industry labs and have been for decades and provide a
good work horse for many analyses.
Similar to AOAC 997.08
Cons/Weaknesses
Supporting Data
#4
FOS-01 Review Forms