AOAC SPIFAN Final Review Forms (March 16, 2016)

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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
2
• Method is straightforward, without complex sample preparation
• Widely available equipment is used
• Considerable reduction in analysis time
Cons/Weaknesses
• Previous remarks were taken up satisfactorily
Supporting Data
General Comment:
• Wide range of matrices tested for 3 types of fructans, including SPIFAN samples
• Multiple labs, analysts, and equipment used
• Performance Characteristics:
Analytical Range: 0.03 – 5 % as-fed, 45-100% ingredient commodity
LOQ: 0.03 % (empirically determined using low spike level in sugar solution)
Accuracy/Recovery: average recoveries within 90 – 110% range
Precision (RSDr): well within acceptability limits
Reproducibility (RSDR ): well within acceptability limits
• System suitability: ok
Recommendation:
Clear method with extensive supporting validation data. We recommend this method for First Action
approval.
FOS-04 Review Forms