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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
1
Infant Formula and Adult Nutrition - Review Form
Evaluation of Method: FOS-04
Title: FOS-04
Author:
Philip Haselberger
Summary of Method:
Method is in 2 parts:-
Part 1:-
- Sample is diluted in water at room temperature and stirred for 30 min
- Sample is then further diluted depending on expected fructan concentration (table provided)
- An aliquot is injected on HPAEC-PAD and the oligosaccharide profile recorded
- Depending on the profile it is possible to identify fructan ingredient present
Part 2:-
- 0.2 g of diluted sample (from above) is treated with sucrase and incubated at 40°C for 2h
- Sodium borohydride is then added and reaction proceeds for 1h at 40°C
- Add acetic acid to stop borohydride reaction
- Add 100ul of internal standard (glucoheptose)
- Add 100ul of furctanase
- Incubate at 40°C for 30min
- Filter and analyse released fructose by HPAEC-PAD
Fructan content is calculated as:-
Fructan = fructose x dilution factor x commodity factor
Where: dilution factor depends on the weight of sample used and amount of dilution
Commodity factor depends on the type of fructan in the sample. The commodity factor varies from 0.953
to 1.23 depedning on the fructan ingredient present.
Method Scope/Applicability:
Method has been applied to the SPIFAN kit (so formula and adult nutritionals) as well as some of Abbots
own samples.
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FOS-04 Review Forms