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FOR EXPERT REVIEW PANEL USE ONLY

NOT FOR DISTRIBUTION

2

Pros/Strengths:

Directly measures fructose liberated and every else is removed.

Cons/Weaknesses

Requires correct identification of raw ingredient to generate a “commodity factor”.

Requires a single source of sucrase (Megazyme)

Potential suppliers of glucoheptose mention but supply may not be available.

Supporting Data

SPIFAN kit (1) used in the validation of the method.

Performance Characteristics:

Analytical Range:

0.03g to 5g/100g

LOQ:

0.03g/100g

Accuracy/Recovery:

Spike recovery of 99.2% (92.9% -108%)within SMPR limits

Precision (RSD

r

):

2.2% RSDr(1.1-3.7%); meets SMPR

Reproducibility (RSD

R

):

System suitability:

No details

Recommendation:

TBD

FOS-04 Review Forms