AOAC SPIFAN Final Review Forms (March 16, 2016)

FOR EXPERT REVIEW PANEL USE ONLY

NOT FOR DISTRIBUTION

Repeatability determined for only 2 SPIFAN matrices for cis and trans lutein and cis and trans b-

carotene –data meet SMPR

Method Clarity:

Method clearly written and should be easy to follow.

Method Safety Concerns:

No safety concerns are noted by the authors

Pros/Strengths:



Well-established methodology



Clearly written and easy to follow



Good chromatography for listed carotenoids



Repeatability and recovery data, though very limited, show that method could meet SMPR



Acceptable linearity

Cons/Weaknesses



Not all carotenoids specified by SMPR are determined by this method



Only SRM 1849a and 2 SPIFAN (?) matrices were analyzed



Not enough validation data

Supporting Data



General Comment:

–

Method Optimization:



Performance Characteristics:

Carot-02 Review Forms