AOAC SPIFAN Final Review Forms (March 16, 2016)

FOR EXPERT REVIEW PANEL USE ONLY

NOT FOR DISTRIBUTION

Analytical Range:

Not clearly specified

LOQ:

Based on spike and recovery data a LOQ of 1.3 μg/100 g was observed for lutein and 1.5 μg/100 g for β-

carotene. SMPR is < 1 ug/100 g but since there is a concentration step in sample preparation LOQ could

probably go down if more sample is taken for evaporation

Accuracy/Recovery:

Limited data but meet SMPR:

Accuracy by spike recovery of SRM1849a at 2 levels of all-trans lutein and all-trans b-carotene –

meet SMPR for recoveries and repeatability (no reference or certified carotenoid values available

from NIST)

Precision (RSD

Limited data but meet SMPR

Reproducibility (RSD

no data available



System suitability:

there is some information on how to determine resolution between cis and trans

isomers but need to specify acceptance criteria

Recommendation:

Expand the scope to include a-carotene and lycopene

Complete validation to determine repeatability and accuracy for different samples

All SPIFAN matrices need to be analyzed

Clarify system suitability requirements

Add data about analytical range

See if LOQ could be improved to meet SMPR

Decision on recommending this method as AOAC First Action is better made after data for more SPIFAN

matrices are collected.

Carot-02 Review Forms