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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
2
Method Safety Concerns:
The authors may need to add safety section to the method which is lacking now particularly with the use of
concentrated nitric acid in microwave.
Pros/Strengths:
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The method is able to analyze choline as well as carnitine simultaneously.
•
The method can analyze choline and carnitione present in free form as well as bound form separately.
•
The method uses internal std for both analytes during extraction as well as analysis.
•
Specific for analytes
•
The method is relatively faster and simple
Cons/Weaknesses
•
The method is not able to differentiate between D & L carnitine.
Supporting Data
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General Comment:
Satisfactory & complete
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Method Optimization:
No method optimization data provided
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Performance Characteristics:
Analytical Range:
The analytical range of the method is from the stated 0.05 to 250 mg/100g choline and 0.05 to 20
mg/100g carnitine.
LOQ:
An LOQ of 0.05 mg/100g was obtained for both free and total choline and carnitine. The LOQ was
calculated from the lowest working standard concentration through the default weights and
dilutions used.
Chol-08 Review Forms