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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
1
Expert Review Panel for Infant Formula and Adult Nutrition
Evaluation of Method GOS-01
Title:
Determination of β-Galactooligosaccharides in Infant
Formula & Adult Nutritionals
Author:
Sean Austin, Denis Cuany, Catherine Murset-Mounoud
Nestlé Research Centre, Vers-Chez-Les-Blanc, 1000 Lausanne, Switzerland
Summary of Method:
Samples are reconstituted in water and further diluted until the concentration of GOS in solution is
appropriate such that the chromatographic signals will be within the range covered by the
standard curve. An aliquot of the sample solution is taken and an internal standard is added, this is
then split into two aliquots. One aliquot is treated with a β-galactosidase the other is not. Then
both aliquots are labelled with a fluorescent tag, 2-aminobenzamide (2AB). After labelling both
aliquots are treated with amyloglucosidase to remove maltodextrins, then they are analysed by
hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection (FLD).
Method Scope/Applicability:
This method is applicable in infant formula and adult nutritional samples.
General comments about the method:
The SLV was performed on the SPIFAN matrix kit containing 19 different matrices, two products
purchased in a Swiss supermarket, and a Nestlé reference sample.
Of the 19 matrices in the SPIFAN kit, 5 were found to contain GOS. Those 5 and the other 3 materials were
analysed on 6 different days in duplicate to determine the precision of the method.
Of the 14 GOS‐free samples, 4 were selected for use in spike/recovery experiments. The four matrices were
spiked with 2 different GOS ingredients at four different levels.
Method Clarity:
The method is clear and well written.
Method Safety Concerns:
Method does not require any especial safety precautions.
Pros/Strengths: