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2. Does the method contain
system suitability tests or
controls as specified by the
SMPR? If not, please indicate
if there is a need for such
tests or controls and which
ones.
Yes.
The proposed method contains injections of blank reagent, working calibration
solutions and control samples.
3. Is there information
demonstrating that the
method system suitability
tests and controls as
specified in the SMPR worked
appropriately and as
expected? If no, please
specify.
Yes
4. Based on the supporting
information, is the method
written clearly and concisely?
If no, please specify the
needed revisions.
Yes
5. Based on the supporting
information, what are the
pros/strengths of the
method?
The Proposed method uses a cation-exchange HPLC separation technique coupled
with post-column ninhydrin
derivatization.
This has been considered as a reference method in term of its robust nature, its
quantitative performance and reproducibility.
Sample components are separated in the column before the reaction, it is not affected
by the sample matrix during reaction with the derivatizing reagent.
6. Based on the supporting
information, what are the
cons/weaknesses of the
method?
Sensitivity may be questionable
Relatively high reagent consumption
The use of UV-visible detection
The purity of the separated peaks should be checked
Only one analyte (theanine) is explored
7. Any general comments
about the method?
No
Do you recommend this
method be adopted as a First
Action and published in the
Official Methods of Analysis
of AOAC INTERNATIONAL?
Please specify rationale.
The proposed method meets the major SMPR criteria and then could be adopted (after
discussion with the ERP members) as a First Action