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Submission Date
2016-07-25 13:07:52
Name
Nour Eddine ES-SAFI
Organization
Mohammed V University in Rabat
Title of Method
Analysis of Theanine in Tea (Camellia sinensis) Dietary Ingredients and Supplements
by HighPerformance Liquid Chromatography with Post-Column Derivatization: Single
Laboratory Validation
AOAC Candidate Method
Number (e.g. ALN-01)
TEA-01
Applicable SMPR
2015.014
Summary:
The Tea-1 method presents experimental technique for Theanine analysis in dietary
ingredients and
supplements using simple buffer extraction followed by cation-exchange
chromatography, post-column
reaction with Ninhydrin reagent and UV/Vis detection
1. Does the applicability of
the method support the
applicability of the SMPR? If
not, please explain what is
missing.
The AOAC SMPR 2015-014 concerns the quantitative determination of catechins,
methyl xanthenes, theaflavins, and theanine. In the proposed Tea-1 method only
theanine is determined.
2. Does the analytical
technique(s) used in the
method meet the SMPR? If
not, please specify how it
differs from what is stated in
the SMPR.
The analytical technique used in the Tea-1 method meet the SMPR. From all the given
results only one does not meet the SMPR. (Recovery: 108.7 % instead of 107 %)
3. Are the definitions
specified in the SMPR used
and applied appropriately in
the method? If no, please
indicate how the terms are
used.
Yes
4. Does the method, as
written, contain all
appropriate precautions and
warnings related to the
method's reagents,
components, instrumentation,
or method steps that may be
hazardous? If no, please
suggest wording or option(s).
Yes
AOAC SPDS ERP - Set 3 Review Form 2