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The Charge
•
Select
B. anthracis
strains representative of the
current available
B. anthracis
collection that
represent the diversity across this species
•
These strains will be used to validate detection
assays
–
A successful assay should detect all of these
isolates*
*Some assays may detect sequences or
materials not present in all isolates
What is a
B. anthracis
isolate?
•
The causative agent of anthrax
•
A bacterium that meets clinical criteria for being
B.
anthracis
–
Colony morphology, Gram stain, presence of capsule,
motility, hemolytic activity,
phage sensitivity, pen
s
, etc.
•
An isolate containing pXO1 and pXO2 unless otherwise
identified as missing one of these plasmids
A i l t th t
b lti l h l
ti
th d
•
n so a e a maps, y mu p e p y ogene c me o s,
to a tight cluster of microbes sharing these
characteristics