20 / 27
FDA/ORA/ORS
LIB #4578
19 of 25
and needed to be within 10%; the ions found were in comparison m/z 174 hence the ion ratios
are as follows: m/z 159 (68%) and 238 (61%). Mitragynine was confirmed in the positive
control and the samples analyzed by the Denver Laboratory. The use of the TSQ provided the
selectivity and sensitivity and mitragynine was not detected in the solvent blank and negative
control.
Figure 15
: a) Mitragynine Solvent Standard - Total Ion Chromatogram (TIC)
b) Mitragynine Reconstructed Ion Chromatogram (RIC)
Kraton_27Dec12_A2 - TIC - SM: 5 RT:
5.00 - 14.98
NL:
1.80E6
6
7
8
9
10
11
12
13
14
Time (min)
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Relative Intensity
RT: 11.53
RT: 12.22
RT: 14.54
13.02 13.56
5.26 5.89
6.87
8.42
9.99
6.36
7.94
9.31
10.90
7.35
RT:
10.50 - 15.00
11
12
13
14
15
Time (min)
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Relative Abundance
Total Ion Chromatogram (TIC)
Sample
Mitragynine Rt=11.53
RT: 11.53
RT: 12.79
RT: 12.39
NL: 8.70E5
TIC F: + c ESI SRM ms2
399.200 [159.099-159.101,
174.099-174.101,
238.199-238.201] MS ICIS
Kraton_28Dec12_A6
RT:
10.50 - 15.00
11
12
13
14
15
Time (min)
0
10
20
30
40
50
60
70
80
90
100
0
10
20
30
40
50
60
70
80
90
100
Relative Abundance
0
10
20
30
40
50
60
70
80
90
100
SRM 399.2-> 174
Sample
Mitragynine Rt=11.53
Sample
Mitragynine
TIC
RT: 12.79 RT: 13.35
RT: 12.27
RT: 14.29
SRM 399.2-> 159.1
Sample
Mitragynine Rt=11.53
RT: 11.53
RT: 12.22 RT: 12.87 RT: 13.81
SRM 399.2-> 238.1
Sample
Mitragynine Rt=11.53
RT: 11.53
RT: 12.44
NL: 3.88E5
m/z= 173.90-174.10 F: + c ESI
SRM ms2 399.200
[159.099-159.101,
174.099-174.101,
238.199-238.201] MS ICIS
Kraton_28Dec12_A6
NL: 2.46E5
m/z= 159.00-159.20 F: + c ESI
SRM ms2 399.200
[159.099-159.101,
174.099-174.101,
238.199-238.201] MS ICIS
Kraton_28Dec12_A6
NL: 2.37E5
m/z= 238.00-238.20 F: + c ESI
SRM ms2 399.200
[159.099-159.101,
174.099-174.101,
238.199-238.201] MS ICIS
Kraton_28Dec12_A6
a
b