21 / 27
FDA/ORA/ORS
LIB #4578
20 of 25
Figure 16:
a) Negative Control - Total Ion Chromatogram (TIC)
b) Negative Control Reconstructed Ion Chromatogram (RIC)
Kraton_27Dec12_A12 - TIC - SM: 5 RT:
5.00 - 14.99
NL:
3.65E2
6
7
8
9
10
11
12
13
14
Time (min)
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Relative Intensity
RT: 11.55
12.24
7.86
10.82
6.95
5.46
10.03
7.45
13.18
5.78
6.65
9.56
14.37
11.12
12.70
8.95
6.29
8.52
10.31
RT:
10.50 - 15.00
11
12
13
14
15
Time (min)
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Relative Abundance
Total Ion Chromatogram (TIC)
Negative Contol
Mitragynine Rt=11.53
11.55
12.24
11.63
10.82
11.51
13.18
11.48
12.32
14.89
12.70
11.12
13.89
12.04
13.98
14.37
13.73
NL: 6.17E2
TIC F: + c ESI SRM ms2
399.200
[159.099-159.101,
174.099-174.101,
238.199-238.201] MS
Kraton_27Dec12_A12
RT:
10.50 - 15.00
11
12
13
14
15
Time (min)
0
10
20
30
40
50
60
70
80
90
100
0
10
20
30
40
50
60
70
80
90
100
Relative Abundance
0
10
20
30
40
50
60
70
80
90
100
SRM 399.2->174.1
Negative Contol
Mitragynine Rt=11.53
11.55
11.12
12.32
11.71
12.70
10.82
11.83
14.61
14.56
13.50 13.99
SRM 399.2->159.1
Negative Contol
Mitragynine Rt=11.53
11.55
11.63
10.82
13.18
11.48
12.32
14.89
13.89
12.04
13.98
13.73
SRM 399.2->238.1
Negative Contol
Mitragynine Rt=11.53
11.63
14.89
13.98
12.04
11.48
13.89 14.37
11.25
13.63
12.95
12.27
13.09
NL: 3.74E2
m/z= 173.90-174.10 F: + c ESI
SRM ms2 399.200
[159.099-159.101,
174.099-174.101,
238.199-238.201] MS
Kraton_27Dec12_A12
NL: 6.17E2
TIC F: + c ESI SRM ms2
399.200 [159.099-159.101,
174.099-174.101,
238.199-238.201] MS
Kraton_27Dec12_A12
NL: 2.52E2
m/z= 238.00-238.20 F: + c ESI
SRM ms2 399.200
[159.099-159.101,
174.099-174.101,
238.199-238.201] MS
Kraton_27Dec12_A12
a
b