49 / 151
Submission Date
2016-09-16 11:57:06
Name
Melanie Downs
Organization
University of Nebraska-Lincoln
Title of Method
Multiplexed LC-MS Method for the Detection and Quantitation of Selected Food
Allergens (milk, hazelnut, peanut and whole egg)
AOAC Candidate Method
Number (e.g. ALN-01)
ALL-02
Applicable SMPR
2016.002
Summary:
The authors of this submission present a method for the LC-MS/MS detection and
quantification of peptides derived from the pepsin-trypsin/chymotrypsin digestion of
food allergens (egg, milk, hazelnut, and peanut) present in the matrices specified in
AOAC SMPR 2016.002.
1. Does the applicability of
the method support the
applicability of the SMPR? If
not, please explain what is
missing.
Yes
2. Does the analytical
technique(s) used in the
method meet the SMPR? If
not, please specify how it
differs from what is stated in
the SMPR.
Yes
3. Are the definitions
specified in the SMPR used
and applied appropriately in
the method? If no, please
indicate how the terms are
used.
No. The authors are unacceptably inconsistent and incomplete with their use of units
throughout the method submission. The use of units for concentrations of food
allergens must be absolutely clear and complete. See comments regarding the
supporting information for further description of issues with units.
4. Does the method, as
written, contain all
appropriate precautions and
warnings related to the
method's reagents,
components, instrumentation,
or method steps that may be
hazardous? If no, please
suggest wording or option(s).
Yes.
AOAC SPSFAM ERP REVIEW: MAIN FORM