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4. Does the method, as
written, contain all
appropriate precautions and
warnings related to the
method's reagents,
components, instrumentation,
or method steps that may be
hazardous? If no, please
suggest wording or option(s).
The information about safety precautions redirect the operator to the consultation of
MSDs for each single reagent used. No other information are provided about this
aspect along the protocol.
1. Are the definitions
specified in the SMPR used
and applied appropriately in
the supporting
documentation (manuscripts,
method studies, etc...)? If not,
please explain the differences
and if the method is impacted
by the difference.
No. In case of the starting material used for spikinf the food matrices (not specified the
form and not taken into consideration the whole allergenic ingredient), the calculation of
the recovery.
2. Is there information
demonstrating that the
method meets the SMPR
Method Performance
Requirements using the
Reference Materials stated in
the SMPR? If not, then
specify what is missing and
how this impacts
demonstration of
performance of the method.
No the RM for allergens were not taken into consideration in this study.
The use of CRM or incurred materials are strongly recommended in order to check
method performance: e.g. recovery, etc.
3. Is there information
demonstrating that the
method performs within the
SMPR Method Performance
REquirements table
specifications for all analytes
in the SMPR applicability
statement? If not, please
specify what is missing and
whether or not the method's
applicability should be
modified.
The results provided in the table are not fully supported by the work performed,
according to what shown and reported in the protocol.
The experimental work carried out has not been properly detailed and some information
are missing.
1. Based on the supporting
information, were there any
additional steps in the
evaluation of the method that
indicated the need for any
additional precautionary
statements in the method?
Yes especially when manipulating strong acids and MMTS (corrosive to tissues)