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ABBOTT NUTRITION DIVISION

TITLE

PAGE NO.

Determination of Biotin in Infant, Pediatric and Adult

Nutritionals by HPLC and Fluorescence Detection

10 of 11

ss: Sample size in g

D

1

: Unit conversion (from per g to per 100 g sample and from ng to

P

g of biotin)

D

1

= (100g/100g)(1

P

g/1000ng) = 1/10 (

P

g/ng/100g)

D

2

: Conversion from sample to reconstituted finish product

For powder: D

2

= 25 g powder

y

(25 g powder + 200 g water) = 25 g

y

225 g = 1/9

For liquid (2 times dilution by weight): D

2

= 2

For ready to feed (RTF, as is): D

2

= 1

3.

Example: Sample Calculation

a)

Analysis of SRM 1849a (1.0020 g)

Sample peak area = 5.8505, C

i

= 40.21 ng/mL, ss = 1.0020 g

Final result: C

p

= C

i

x 50

y

ss x D

1

x D

2

= 40.21 (ng/mL) x 50 (mL)

y

1.0020 x (1/10) (

P

g/ng/100g)

x (1/9) = 22.29

P

g/100g reconstituted finish product

b)

Analysis of Infant Formula RTF (20.0333 g)

Sample peak area = 1.7494, C

i

= 15.78 ng/mL, ss = 20.0333 g

Final result: C

p

= C

i

x 50

y

ss x D

1

x D

2

= 15.78 (ng/mL) x 50 (mL)

y

20.0333 x (1/10) (

P

g/ng/100g)

x 1 = 3.94

P

g/100g RTF

Bio-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE